qRT-PCR for enterovirus detection: Conversion to ultrafast protocols
نویسندگان
چکیده
منابع مشابه
DEVELOPMENT OF QRT-PCR PROTOCOLS FOR RAPID XYLELLA FASTIDIOSA SUBSPECIES DIAGNOSTICS Principal Investigator:
Xylella fastidiosa (Xf) is a plant pathogenic bacterium that is transmitted between hosts by the glassy-winged sharpshooter (GWSS; Homalodisca vitripennis). Multiple subspecies of Xf occur and are host specific. Xf subsp. fastidiosa is the causal agent of Pierce’s disease (PD). Xf subsp. multiplex and Xf subsp. sandyi are commonly found in North America but do not cause PD. Rapid diagnostics to...
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Various methods for the recovery and detection of HAV have been suggested, and molecular tests have recently provided an effective replacement for the traditional methods. Real-time RT-PCR technology offers many advantages over conventional RT-PCR in terms of rapidity and specificity. Most procedures are based on the TaqMan chemistry, but some researchers have used the SYBR Green I approach, wh...
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1Bacterial resistance to antibiotics currently represents a topic of high interest. Unfortunately, studies addressing their adaptation and multidrug tolerance latency are not sufficient for a comprehensive description of the pathogen-host molecular interaction mechanisms (interactome) and the bacterial internalization into human cells, which leads to modified human genes. Bacterial persisters a...
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ژورنال
عنوان ژورنال: Journal of King Saud University - Science
سال: 2018
ISSN: 1018-3647
DOI: 10.1016/j.jksus.2017.04.003